DEOXYRIBONUCLEASE HPAII Meaning and
Definition
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Deoxyribonuclease HpaII is a type II restriction enzyme that recognizes and cleaves DNA at specific sequences. It is derived from the bacteria Haemophilus parainfluenzae and is named after this organism. This enzyme is classified as a restriction enzyme due to its ability to restrict the growth of invading foreign DNA, such as bacteriophages.
Deoxyribonuclease HpaII specifically recognizes a palindromic DNA sequence, which is a sequence that reads the same backwards and forwards. This sequence is known as 5'-CCGG-3'. HpaII binds to this specific sequence and cleaves the DNA between the second cytosine (C) and the first guanine (G) residue on both DNA strands, generating two smaller DNA fragments. The cleavage site of HpaII is within the recognition sequence, resulting in the formation of blunt ends.
The activity of Deoxyribonuclease HpaII is dependent on various factors such as the salt concentration, pH, and temperature. It functions optimally under specific conditions and can exhibit exonuclease activity in the absence of magnesium ions. This enzyme can be used in various molecular biology techniques, such as DNA digestion, restriction mapping, and DNA sequencing.
In summary, Deoxyribonuclease HpaII is a restriction enzyme that specifically recognizes and cleaves DNA at the palindromic sequence 5'-CCGG-3'. Its ability to cleave DNA at specific sites makes it a valuable tool in molecular biology research and applications.
Common Misspellings for DEOXYRIBONUCLEASE HPAII
- seoxyribonuclease hpaii
- xeoxyribonuclease hpaii
- ceoxyribonuclease hpaii
- feoxyribonuclease hpaii
- reoxyribonuclease hpaii
- eeoxyribonuclease hpaii
- dwoxyribonuclease hpaii
- dsoxyribonuclease hpaii
- ddoxyribonuclease hpaii
- droxyribonuclease hpaii
- d4oxyribonuclease hpaii
- d3oxyribonuclease hpaii
- deixyribonuclease hpaii
- dekxyribonuclease hpaii
- delxyribonuclease hpaii
- depxyribonuclease hpaii
- de0xyribonuclease hpaii
- de9xyribonuclease hpaii
- deozyribonuclease hpaii
- deocyribonuclease hpaii
Etymology of DEOXYRIBONUCLEASE HPAII
The word "Deoxyribonuclease HpaII" is derived from its function and its source of origin.
"Deoxyribonuclease" is a compound word consisting of three components: "deoxyribo-", "nucleo-", and "-ase".
- "Deoxyribo-" refers to deoxyribose, a type of sugar found in DNA.
- "Nucleo-" refers to nucleic acid, which DNA is composed of.
- "-ase" is a suffix commonly used in biochemistry to indicate an enzyme.
So, "Deoxyribonuclease" literally means an enzyme that acts upon deoxyribonucleic acid (DNA) by cleaving its strands.
The second part, "HpaII", refers to the specific restriction enzyme that this particular deoxyribonuclease is derived from.
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