CDNA ARRAY Meaning and
Definition
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A cDNA array, also known as a complementary DNA array, is a powerful tool used in molecular biology to analyze gene expression patterns and identify specific genes that are active or inactive in a given sample. It is a high-throughput technique that allows researchers to simultaneously examine thousands of genes in a single experiment.
The process of creating a cDNA array involves immobilizing a large number of DNA fragments, called cDNAs, onto a solid surface such as a microarray slide or a glass chip. These cDNAs are synthesized from mRNAs present in the sample using an enzyme called reverse transcriptase.
Once the cDNAs are immobilized, they can be hybridized with fluorescently labeled DNA or RNA probes that represent the genes of interest. The probes will specifically bind to the complementary cDNAs on the array, and the level of fluorescent signal can be quantified.
By measuring the fluorescence intensity of each cDNA spot on the array, scientists can determine the relative abundance of corresponding mRNAs in the sample. This information provides insights into gene expression patterns, allowing researchers to identify genes that are upregulated or downregulated under different conditions or in different cell types.
cDNA arrays have proven to be an invaluable tool in studying complex biological processes, including development, disease progression, and drug responses. They offer a comprehensive view of gene expression patterns and allow researchers to identify potential targets for further investigation or therapeutic intervention.
Common Misspellings for CDNA ARRAY
- xdna array
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Etymology of CDNA ARRAY
The word "cDNA array" is derived from two main components: "cDNA" and "array".
1. cDNA: cDNA stands for complementary DNA. It refers to a type of DNA molecule that is synthesized from a messenger RNA (mRNA) template through a process called reverse transcription. This synthesis involves the use of an enzyme called reverse transcriptase. cDNA is often used in molecular biology research to study gene expression and analyze the transcriptome.
2. Array: An array refers to a systematic arrangement or organization of objects or elements in a specific pattern. In the context of molecular biology, an array typically refers to a collection of many spots or locations on a surface, where each spot contains a distinct biological molecule, such as DNA or cDNA. These spots are often arranged in a grid-like pattern, allowing for parallel analysis of multiple molecules simultaneously.
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